Naša qPCR odličnost
Znanje in izkušnje so ključ delavnic qPCR Experience podjetja BioSistemika’s, čigar korenine izhajajo iz prvega in najbolj izkušenega qPCR centra v Sloveniji - Nacionalnega inštituta za biologijo, kjer smo še zmeraj vpeti v raziskovalno delo na področju qPCR in mikromrež. Od leta 2002 smo prisotni tudi v mednarodni qPCR javnosti, kar je razvidno iz spodaj navedenih znanstvenih objav.
Praktične izkušnje iz področja znanosti, diagnostike, industrijskih raziskav ter odlične sposobnosti podajanja znanja tako mednarodni znanstveni, strokovni javnosti, mednarodnim združenjem kot tudi v okviru notranjih izobraževanj v podjetjih, bodo zagotovile visoko kakovost naših delavnic. Tako bodo področja kot so PCR v realnem času in z njim povezano raziskovanje ter reševanje težav pri delu v laboratoriju zagotovo predstavljena na jasen in profesionalen način.
Naši strokovnjaki so že od leta 2006 vključeni v organizacijo praktičnih delavnic qPCR za domače in mednarodne partnerje Nacionalnega inštituta za biologijo.
Reference naših strokovnjakov
ČEPIN Urška, GUTIERREZ-AGUIRRE Ion, BALAŽIC Leonida, POMPE NOVAK Maruša, GRUDEN Kristina, RAVNIKAR Maja. A one-step reverse transcription real-time PCR assay for the detection and quantitation of Grapevine fanleaf virus. J. virol. methods., 2010, vol. 170, issues 1-2, p. 47-56. http://dx.doi.org/10.1016/j.jviromet.2010.08.018
PAPAZOVA Nina, ZHANG David, GRUDEN Kristina, VOJVODA Jana, YANG Litao, BUH GAŠPARIČ Meti, BLEJEC Andrej, FOUILLOUX Stephane, DE LOOSE Marc, TAVERNIERS Isabel. Evaluation of the reliability of maize reference assays for GMO quantification. Anal. bioanal. chem., 2010, vol. 396, no. 6, p. 2189-2201. http://dx.doi.org/10.1007/s00216-009-3386-4
BAEBLER Špela, KREČIČ STRES Hana, ROTTER Ana, KOGOVŠEK Polona, CANKAR Katarina, KOK Esther, GRUDEN Kristina, KOVAČ Maja, ŽEL Jana, POMPE NOVAK Maruša, RAVNIKAR Maja. PVYNTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation. Mol. plant pathol., 2009, vol. 10, no. 2, p. 263-275. http://dx.doi.org/10.1111/J.1364-3703.2008.00530.X
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460, p. 38. http://www.biomedcentral.com/1471-2164/10/460/abstract
VIRANT-KLUN Irma, VOGLER Andrej, ŠINKOVEC Jasna, HREN Matjaž, GRUDEN Kristina, MALIČEV Elvira, MEDEN-VRTOVEC Helena. Ovarijska neplodnost, matične celice in oogeneza in vitro = Ovarian infertility, stem cells and oogenesis in vitro. In: 4. Kongres ginekologov in porodničarjev Slovenije z mednarodno udeležbo, Portorož, 11. do 13. november 2009, (Zdravniški Vestnik, vol. 78, Suppl 1). Ljubljana: Slovensko zdravniško društvo, 2009, p. I-57 - I-61. http://szd.si/user_files/vsebina/Zdravniski_Vestnik/2009/Suplement/057-061.pdf (publication is in Slovene language)
KOGOVŠEK Polona, GOW Lisa, POMPE NOVAK Maruša, GRUDEN Kristina, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Single-step RT real-time PCR for sensitive detection and discrimination of potato virus Y isolates. J. virol. methods., 2008, vol. 149, no. 1, p. 1-11.
GUTIERREZ-AGUIRRE Ion, STEYER Andrej, BOBEN Jana, GRUDEN Kristina, POLJŠAK-PRIJATELJ Mateja, RAVNIKAR Maja. Sensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay. J. Clin. Microbiol., 2008, vol. 46, no. 8, p. 2547-2554.
ŽEL Jana, MAZZARA Marco, SAVINI Cristian, CORDEIL Stephane, CAMLOH Marjana, ŠTEBIH Dejan, CANKAR Katarina, GRUDEN Kristina, MORISSET Dany, EEDE G. van den. Method validation and quality management in the flexible scope of accreditation : an example of laboratories testing for genetically modified organisms. Food analytical methods, 2008, issue 2, vol. 1, p. 61-72. http://dx.doi.org/10.1007/s12161-008-9016-5
BUH GAŠPARIČ Meti, CANKAR Katarina, ŽEL Jana, GRUDEN Kristina. Comparision of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms. BMC Biotechnol, 2008, vol. 8, no. 26, p. 1-12. http://dx.doi.org/10.1186/1472-6750-8-26
HREN Matjaž, BOBEN Jana, ROTTER Ana, KRALJ NOVAK Petra, GRUDEN Kristina, RAVNIKAR Maja. Real-time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine : comparision with conventional PCR detection and application in diagnostics. Plant Pathol., 2007, vol. 56, p. 785-796.
CANKAR Katarina, ŠTEBIH Dejan, DREO Tanja, ŽEL Jana, GRUDEN Kristina. Critical points of DNA quantification by real-time PCR-effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol, 2006, vol. 6, no. 37.
CANKAR Katarina, RAVNIKAR Maja, ŽEL Jana, GRUDEN Kristina, TOPLAK Nataša. Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J. AOAC Int., 2005, vol. 88, no. 3, p. 814-822.
GRUDEN Kristina, POMPE NOVAK Maruša, BAEBLER Špela, KREČIČ STRES Hana, TOPLAK Nataša, HREN Matjaž, KOGOVŠEK Polona, GOW Lisa, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Expression microarrays in plant-virus interaction. In: FOSTER, Gary D. (ed.). Plant virology protocols: from viral sequence to protein function, (Methods in molecular biology, 451). 2nd ed. Totowa: Humana Press, 2008, p. 583-613.
HREN Matjaž, DREO Tanja, ERJAVEC Jana, NIKOLIĆ Petra, BOBEN Jana, GRUDEN Kristina, DERMASTIA Marina, CAMLOH Marjana, RAVNIKAR Maja. Real-time PCR detection methods for economically important grapevine related bacteria. In: DELROT, Serge (ed.), MEDRANO, Hipólito (ed.), OR, Etti (ed.), BAVARESCO, Luigi (ed.), GRANDO, Stella (ed.). Methodologies and results in grapevine research. Dordrecht: Springer, 2010, p. 229-246.
Praktične izkušnje iz področja znanosti, diagnostike, industrijskih raziskav ter odlične sposobnosti podajanja znanja tako mednarodni znanstveni, strokovni javnosti, mednarodnim združenjem kot tudi v okviru notranjih izobraževanj v podjetjih, bodo zagotovile visoko kakovost naših delavnic. Tako bodo področja kot so PCR v realnem času in z njim povezano raziskovanje ter reševanje težav pri delu v laboratoriju zagotovo predstavljena na jasen in profesionalen način.
Naši strokovnjaki so že od leta 2006 vključeni v organizacijo praktičnih delavnic qPCR za domače in mednarodne partnerje Nacionalnega inštituta za biologijo.
Spodnja shema prikazuje celoten prikaz dela, ki se nanaša na analizo izražanja genov s qPCR (PCR v realnem času), ene izmed najbolj uporabnih aplikacij qPCR. Obsega več stopenj, za vsako pa je potreben specifičen nabor vrlin. Na naših delavnicah vas bodo naši strokovnjaki seznanili s kritičnimi točkami v vseh korakih, podali rešitve in se o njih z vami pogovorili.
Reference naših strokovnjakov
ČEPIN Urška, GUTIERREZ-AGUIRRE Ion, BALAŽIC Leonida, POMPE NOVAK Maruša, GRUDEN Kristina, RAVNIKAR Maja. A one-step reverse transcription real-time PCR assay for the detection and quantitation of Grapevine fanleaf virus. J. virol. methods., 2010, vol. 170, issues 1-2, p. 47-56. http://dx.doi.org/10.1016/j.jviromet.2010.08.018
PAPAZOVA Nina, ZHANG David, GRUDEN Kristina, VOJVODA Jana, YANG Litao, BUH GAŠPARIČ Meti, BLEJEC Andrej, FOUILLOUX Stephane, DE LOOSE Marc, TAVERNIERS Isabel. Evaluation of the reliability of maize reference assays for GMO quantification. Anal. bioanal. chem., 2010, vol. 396, no. 6, p. 2189-2201. http://dx.doi.org/10.1007/s00216-009-3386-4
BAEBLER Špela, KREČIČ STRES Hana, ROTTER Ana, KOGOVŠEK Polona, CANKAR Katarina, KOK Esther, GRUDEN Kristina, KOVAČ Maja, ŽEL Jana, POMPE NOVAK Maruša, RAVNIKAR Maja. PVYNTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation. Mol. plant pathol., 2009, vol. 10, no. 2, p. 263-275. http://dx.doi.org/10.1111/J.1364-3703.2008.00530.X
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460, p. 38. http://www.biomedcentral.com/1471-2164/10/460/abstract
VIRANT-KLUN Irma, VOGLER Andrej, ŠINKOVEC Jasna, HREN Matjaž, GRUDEN Kristina, MALIČEV Elvira, MEDEN-VRTOVEC Helena. Ovarijska neplodnost, matične celice in oogeneza in vitro = Ovarian infertility, stem cells and oogenesis in vitro. In: 4. Kongres ginekologov in porodničarjev Slovenije z mednarodno udeležbo, Portorož, 11. do 13. november 2009, (Zdravniški Vestnik, vol. 78, Suppl 1). Ljubljana: Slovensko zdravniško društvo, 2009, p. I-57 - I-61. http://szd.si/user_files/vsebina/Zdravniski_Vestnik/2009/Suplement/057-061.pdf (publication is in Slovene language)
KOGOVŠEK Polona, GOW Lisa, POMPE NOVAK Maruša, GRUDEN Kristina, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Single-step RT real-time PCR for sensitive detection and discrimination of potato virus Y isolates. J. virol. methods., 2008, vol. 149, no. 1, p. 1-11.
GUTIERREZ-AGUIRRE Ion, STEYER Andrej, BOBEN Jana, GRUDEN Kristina, POLJŠAK-PRIJATELJ Mateja, RAVNIKAR Maja. Sensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay. J. Clin. Microbiol., 2008, vol. 46, no. 8, p. 2547-2554.
ŽEL Jana, MAZZARA Marco, SAVINI Cristian, CORDEIL Stephane, CAMLOH Marjana, ŠTEBIH Dejan, CANKAR Katarina, GRUDEN Kristina, MORISSET Dany, EEDE G. van den. Method validation and quality management in the flexible scope of accreditation : an example of laboratories testing for genetically modified organisms. Food analytical methods, 2008, issue 2, vol. 1, p. 61-72. http://dx.doi.org/10.1007/s12161-008-9016-5
BUH GAŠPARIČ Meti, CANKAR Katarina, ŽEL Jana, GRUDEN Kristina. Comparision of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms. BMC Biotechnol, 2008, vol. 8, no. 26, p. 1-12. http://dx.doi.org/10.1186/1472-6750-8-26
HREN Matjaž, BOBEN Jana, ROTTER Ana, KRALJ NOVAK Petra, GRUDEN Kristina, RAVNIKAR Maja. Real-time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine : comparision with conventional PCR detection and application in diagnostics. Plant Pathol., 2007, vol. 56, p. 785-796.
CANKAR Katarina, ŠTEBIH Dejan, DREO Tanja, ŽEL Jana, GRUDEN Kristina. Critical points of DNA quantification by real-time PCR-effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol, 2006, vol. 6, no. 37.
CANKAR Katarina, RAVNIKAR Maja, ŽEL Jana, GRUDEN Kristina, TOPLAK Nataša. Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J. AOAC Int., 2005, vol. 88, no. 3, p. 814-822.
GRUDEN Kristina, POMPE NOVAK Maruša, BAEBLER Špela, KREČIČ STRES Hana, TOPLAK Nataša, HREN Matjaž, KOGOVŠEK Polona, GOW Lisa, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Expression microarrays in plant-virus interaction. In: FOSTER, Gary D. (ed.). Plant virology protocols: from viral sequence to protein function, (Methods in molecular biology, 451). 2nd ed. Totowa: Humana Press, 2008, p. 583-613.
HREN Matjaž, DREO Tanja, ERJAVEC Jana, NIKOLIĆ Petra, BOBEN Jana, GRUDEN Kristina, DERMASTIA Marina, CAMLOH Marjana, RAVNIKAR Maja. Real-time PCR detection methods for economically important grapevine related bacteria. In: DELROT, Serge (ed.), MEDRANO, Hipólito (ed.), OR, Etti (ed.), BAVARESCO, Luigi (ed.), GRANDO, Stella (ed.). Methodologies and results in grapevine research. Dordrecht: Springer, 2010, p. 229-246.